{"id":109866,"date":"2026-06-08T11:41:53","date_gmt":"2026-06-08T06:11:53","guid":{"rendered":"https:\/\/staging.nanoschool.in\/wp\/course\/a-hands-on-guide-using-crisprdirect-and-chopchop\/"},"modified":"2026-06-23T11:31:55","modified_gmt":"2026-06-23T06:01:55","slug":"crispr-guide-design-using-crisprdirect-and-chopchop","status":"publish","type":"product","link":"https:\/\/staging.nanoschool.in\/wp\/course\/crispr-guide-design-using-crisprdirect-and-chopchop\/","title":{"rendered":"A Hands-On Guide Using CRISPRdirect and CHOPCHOP"},"content":{"rendered":"<div style=\"font-family: 'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; line-height: 1.6; color: #2d3748; max-width: 900px; margin: 0 auto; padding: 40px 20px; background-color: #ffffff;\">\n<div style=\"display: grid; grid-template-columns: 1fr 2fr; gap: 1px; background-color: #e2e8f0; border: 1px solid #e2e8f0; border-radius: 8px; overflow: hidden; margin-bottom: 40px; box-shadow: 0 4px 6px rgba(0,0,0,0.05);\">\n<div style=\"background-color: #f7fafc; padding: 12px 20px; font-weight: bold; color: #2c5282;\">Attribute<\/div>\n<div style=\"background-color: #ffffff; padding: 12px 20px; font-weight: bold; color: #2c5282;\">Detail<\/div>\n<div style=\"background-color: #f7fafc; padding: 10px 20px; border-top: 1px solid #e2e8f0;\">Format<\/div>\n<div style=\"background-color: #ffffff; padding: 10px 20px; border-top: 1px solid #e2e8f0;\">Recorded Lectures (Self-Paced)<\/div>\n<div style=\"background-color: #f7fafc; padding: 10px 20px; border-top: 1px solid #e2e8f0;\">Level<\/div>\n<div style=\"background-color: #ffffff; padding: 10px 20px; border-top: 1px solid #e2e8f0;\">Intermediate<\/div>\n<div style=\"background-color: #f7fafc; padding: 10px 20px; border-top: 1px solid #e2e8f0;\">Duration<\/div>\n<div style=\"background-color: #ffffff; padding: 10px 20px; border-top: 1px solid #e2e8f0;\">3 Days (1.5 hours per day)<\/div>\n<div style=\"background-color: #f7fafc; padding: 10px 20px; border-top: 1px solid #e2e8f0;\">Certification<\/div>\n<div style=\"background-color: #ffffff; padding: 10px 20px; border-top: 1px solid #e2e8f0;\">e-Certification + e-Marksheet<\/div>\n<div style=\"background-color: #f7fafc; padding: 10px 20px; border-top: 1px solid #e2e8f0;\">Tools<\/div>\n<div style=\"background-color: #ffffff; padding: 10px 20px; border-top: 1px solid #e2e8f0;\">Benchling, CRISPOR, Cas-OFFinder, SnapGene, Addgene, NCBI Primer-BLAST<\/div>\n<\/div>\n<h3 style=\"font-size: 24px; font-weight: bold; color: #2c5282; margin-bottom: 15px;\">About the A Hands Course<\/h3>\n<div style=\"margin-bottom: 20px;\">CRISPR technology has revolutionized the field of genetic research, making genome editing more accessible and precise. This 3-day course is designed to offer an in-depth, hands-on guide to using CRISPRdirect and CHOPCHOP, two powerful tools for designing CRISPR experiments.<\/div>\n<div style=\"margin-bottom: 30px;\">Participants will learn how to effectively utilize these tools to create guide RNA (gRNA) for targeting specific genes, perform on-target and off-target analysis, and interpret results for different research applications. The course will include real-time demonstrations, case studies, and interactive sessions to ensure participants gain the practical skills required for successful implementation of CRISPR technology in various biological systems. By the end of the course, attendees will be equipped to independently conduct CRISPR experiments and troubleshoot common issues associated with gRNA design and target specificity.<\/div>\n<div style=\"background-color: #1a365d; color: white; padding: 30px; border-radius: 8px; margin-bottom: 40px;\">\n<h3 style=\"font-size: 22px; font-weight: bold; margin-bottom: 15px; color: white;\">Program Highlights<\/h3>\n<div style=\"display: grid; grid-template-columns: 1fr 1fr; gap: 20px;\">\n<div style=\"font-size: 14px;\">\u2022 Comprehensive coverage of A Hands from fundamentals to advanced applications<\/div>\n<div style=\"font-size: 14px;\">\u2022 Hands-on projects and real-world case studies in CRISPR &amp; Gene Editing<\/div>\n<div style=\"font-size: 14px;\">\u2022 Expert-curated curriculum aligned with current industry standards<\/div>\n<div style=\"font-size: 14px;\">\u2022 Access to recorded lectures and e-LMS platform for flexible, self-paced learning<\/div>\n<div style=\"font-size: 14px;\">\u2022 e-Certification and e-Marksheet upon successful completion<\/div>\n<div style=\"font-size: 14px;\">\u2022 Dedicated mentor support and interactive doubt-clearing sessions<\/div>\n<div style=\"font-size: 14px;\">\u2022 Exposure to industry-standard tools and platforms used in CRISPR &amp; Gene Editing<\/div>\n<div style=\"font-size: 14px;\">\u2022 Career-oriented training for academic and professional growth in CRISPR &amp; Gene Editing<\/div>\n<\/div>\n<\/div>\n<h3 style=\"font-size: 24px; font-weight: bold; color: #2c5282; margin-bottom: 20px;\">Course Curriculum<\/h3>\n<div style=\"margin-bottom: 40px;\">\n<div style=\"border: 1px solid #e2e8f0; border-radius: 6px; padding: 20px; margin-bottom: 15px; background-color: #fcfcfc;\">\n<h4 style=\"font-weight: bold; color: #3182ce; margin-bottom: 10px; font-size: 18px;\">Module 1: Introduction to A Hands<\/h4>\n<ul style=\"font-size: 14px; margin: 0; padding-left: 18px; color: #4a5568;\">\n<li>Overview and historical evolution of A Hands<\/li>\n<li>Key terminology, definitions, and core concepts in CRISPR &amp; Gene Editing<\/li>\n<li>Current industry landscape, trends, and career opportunities<\/li>\n<li>Setting up the learning environment and essential tools<\/li>\n<\/ul>\n<\/div>\n<div style=\"border: 1px solid #e2e8f0; border-radius: 6px; padding: 20px; margin-bottom: 15px; background-color: #fcfcfc;\">\n<h4 style=\"font-weight: bold; color: #3182ce; margin-bottom: 10px; font-size: 18px;\">Module 2: Fundamentals and Theoretical Foundations<\/h4>\n<ul style=\"font-size: 14px; margin: 0; padding-left: 18px; color: #4a5568;\">\n<li>Core principles and scientific\/theoretical underpinnings of A Hands<\/li>\n<li>Mathematical and analytical frameworks relevant to CRISPR &amp; Gene Editing<\/li>\n<li>Comparative analysis of major approaches and methodologies<\/li>\n<li>Understanding key standards, guidelines, and best practices<\/li>\n<\/ul>\n<\/div>\n<div style=\"border: 1px solid #e2e8f0; border-radius: 6px; padding: 20px; margin-bottom: 15px; background-color: #fcfcfc;\">\n<h4 style=\"font-weight: bold; color: #3182ce; margin-bottom: 10px; font-size: 18px;\">Module 3: On Guide Using CRISPRdirect and CHOPCHOP<\/h4>\n<ul style=\"font-size: 14px; margin: 0; padding-left: 18px; color: #4a5568;\">\n<li>Core concepts and techniques in On Guide Using CRISPRdirect and CHOPCHOP<\/li>\n<li>Practical implementation and hands-on exercises<\/li>\n<li>Integration of On Guide Using CRISPRdirect and CHOPCHOP with A Hands workflows<\/li>\n<li>Case study: Real-world application of On Guide Using CRISPRdirect and CHOPCHOP<\/li>\n<\/ul>\n<\/div>\n<div style=\"border: 1px solid #e2e8f0; border-radius: 6px; padding: 20px; margin-bottom: 15px; background-color: #fcfcfc;\">\n<h4 style=\"font-weight: bold; color: #3182ce; margin-bottom: 10px; font-size: 18px;\">Module 4: Cas9 Mechanisms<\/h4>\n<ul style=\"font-size: 14px; margin: 0; padding-left: 18px; color: #4a5568;\">\n<li>Introduction to Cas9 Mechanisms concepts and methodologies<\/li>\n<li>Step-by-step practical implementation of Cas9 Mechanisms techniques<\/li>\n<li>Tools and platforms commonly used for Cas9 Mechanisms<\/li>\n<li>Troubleshooting, optimization, and best practices<\/li>\n<\/ul>\n<\/div>\n<div style=\"border: 1px solid #e2e8f0; border-radius: 6px; padding: 20px; margin-bottom: 15px; background-color: #fcfcfc;\">\n<h4 style=\"font-weight: bold; color: #3182ce; margin-bottom: 10px; font-size: 18px;\">Module 5: Guide RNA Design<\/h4>\n<ul style=\"font-size: 14px; margin: 0; padding-left: 18px; color: #4a5568;\">\n<li>Introduction to Guide RNA Design concepts and methodologies<\/li>\n<li>Step-by-step practical implementation of Guide RNA Design techniques<\/li>\n<li>Tools and platforms commonly used for Guide RNA Design<\/li>\n<li>Troubleshooting, optimization, and best practices<\/li>\n<\/ul>\n<\/div>\n<div style=\"border: 1px solid #e2e8f0; border-radius: 6px; padding: 20px; margin-bottom: 15px; background-color: #fcfcfc;\">\n<h4 style=\"font-weight: bold; color: #3182ce; margin-bottom: 10px; font-size: 18px;\">Module 6: Advanced Topics and Emerging Trends in CRISPR &amp; Gene Editing<\/h4>\n<ul style=\"font-size: 14px; margin: 0; padding-left: 18px; color: #4a5568;\">\n<li>Cutting-edge research and innovations in A Hands<\/li>\n<li>Integration with AI, automation, and modern technologies<\/li>\n<li>Industry case studies and real-world problem solving<\/li>\n<li>Future directions and career pathways in CRISPR &amp; Gene Editing<\/li>\n<\/ul>\n<\/div>\n<div style=\"border: 1px solid #e2e8f0; border-radius: 6px; padding: 20px; margin-bottom: 15px; background-color: #fcfcfc;\">\n<h4 style=\"font-weight: bold; color: #3182ce; margin-bottom: 10px; font-size: 18px;\">Module 7: Capstone Project and Assessment<\/h4>\n<ul style=\"font-size: 14px; margin: 0; padding-left: 18px; color: #4a5568;\">\n<li>End-to-end project implementation using A Hands skills<\/li>\n<li>Peer review, collaborative exercises, and expert feedback<\/li>\n<li>Portfolio-ready project documentation and presentation<\/li>\n<li>Final assessment and course completion evaluation<\/li>\n<\/ul>\n<\/div>\n<\/div>\n<div style=\"background-color: #f7fafc; border-radius: 8px; padding: 30px; margin-bottom: 40px;\">\n<h3 style=\"font-size: 20px; font-weight: bold; color: #2c5282; margin-bottom: 15px;\">Tools, Techniques, or Platforms Covered<\/h3>\n<div style=\"display: flex; flex-wrap: wrap; gap: 10px;\"><span style=\"background: white; padding: 5px 15px; border: 1px solid #cbd5e0; border-radius: 20px; font-size: 13px;\">Benchling<\/span><br \/>\n<span style=\"background: white; padding: 5px 15px; border: 1px solid #cbd5e0; border-radius: 20px; font-size: 13px;\">CRISPOR<\/span><br \/>\n<span style=\"background: white; padding: 5px 15px; border: 1px solid #cbd5e0; border-radius: 20px; font-size: 13px;\">Cas-OFFinder<\/span><br \/>\n<span style=\"background: white; padding: 5px 15px; border: 1px solid #cbd5e0; border-radius: 20px; font-size: 13px;\">SnapGene<\/span><br \/>\n<span style=\"background: white; padding: 5px 15px; border: 1px solid #cbd5e0; border-radius: 20px; font-size: 13px;\">Addgene<\/span><br \/>\n<span style=\"background: white; padding: 5px 15px; border: 1px solid #cbd5e0; border-radius: 20px; font-size: 13px;\">NCBI Primer-BLAST<\/span><\/div>\n<\/div>\n<h3 style=\"font-size: 24px; font-weight: bold; color: #2c5282; margin-bottom: 15px;\">Real-World Applications<\/h3>\n<div style=\"margin-bottom: 30px;\">\n<ul style=\"padding-left: 20px; margin-top: 10px;\">\n<li>Apply A Hands skills directly to academic research, thesis work, and publications<\/li>\n<li>Build a professional portfolio showcasing practical CRISPR &amp; Gene Editing competencies<\/li>\n<li>Solve industry-relevant problems using A Hands methodologies and tools<\/li>\n<li>Contribute to open-source projects and collaborative research in CRISPR &amp; Gene Editing<\/li>\n<li>Prepare for competitive examinations, interviews, and professional certifications in CRISPR &amp; Gene Editing<\/li>\n<\/ul>\n<\/div>\n<h3 style=\"font-size: 24px; font-weight: bold; color: #2c5282; margin-bottom: 15px;\">Who Should Attend &amp; Prerequisites<\/h3>\n<div style=\"margin-bottom: 30px;\">\n<ul style=\"padding-left: 20px; margin-top: 10px;\">\n<li>Students pursuing degrees in CRISPR &amp; Gene Editing, science, engineering, or related disciplines<\/li>\n<li>Working professionals seeking to upskill or transition into CRISPR &amp; Gene Editing roles<\/li>\n<li>Researchers and academicians looking to adopt modern techniques in CRISPR &amp; Gene Editing<\/li>\n<li>Entrepreneurs, freelancers, and self-learners interested in practical CRISPR &amp; Gene Editing knowledge<\/li>\n<\/ul>\n<p><strong>Prerequisites:<\/strong> Some familiarity with basic concepts in CRISPR &amp; Gene Editing will be helpful but is not mandatory. A willingness to learn and engage with hands-on exercises is essential.<\/p>\n<\/div>\n<h3 style=\"font-size: 24px; font-weight: bold; color: #2c5282; margin-bottom: 20px;\">Frequently Asked Questions<\/h3>\n<div style=\"margin-bottom: 40px;\">\n<div style=\"margin-bottom: 20px;\">\n<div style=\"font-weight: bold; color: #2d3748;\">1. What is the format of this A Hands-On Guide Using CRISPRdirect and CHOPCHOP course?<\/div>\n<div style=\"color: #4a5568; font-size: 15px;\">This is an Recorded Lectures (Self-Paced) course delivered via our e-LMS platform. You will have access to pre-recorded video lectures, reading materials, assignments, quizzes, and hands-on projects that you can complete at your own pace.<\/div>\n<\/div>\n<div style=\"margin-bottom: 20px;\">\n<div style=\"font-weight: bold; color: #2d3748;\">2. Will I receive a certificate after completing this course?<\/div>\n<div style=\"color: #4a5568; font-size: 15px;\">Yes! Upon successful completion of all modules, assignments, and assessments, you will receive an e-Certification along with an e-Marksheet from NanoSchool (NSTC) that you can showcase on your CV and LinkedIn profile.<\/div>\n<\/div>\n<div style=\"margin-bottom: 20px;\">\n<div style=\"font-weight: bold; color: #2d3748;\">3. What are the prerequisites for this course?<\/div>\n<div style=\"color: #4a5568; font-size: 15px;\">Learners should have a foundational understanding of CRISPR &amp; Gene Editing concepts. Familiarity with basic tools and programming is recommended.<\/div>\n<\/div>\n<div style=\"margin-bottom: 20px;\">\n<div style=\"font-weight: bold; color: #2d3748;\">4. How long will I have access to the course materials?<\/div>\n<div style=\"color: #4a5568; font-size: 15px;\">You will have access to all course materials for the duration of 3 Days (1.5 hours per day). The self-paced format allows you to learn according to your own schedule through our online learning management system.<\/div>\n<\/div>\n<div style=\"margin-bottom: 20px;\">\n<div style=\"font-weight: bold; color: #2d3748;\">5. Is mentor support available during the course?<\/div>\n<div style=\"color: #4a5568; font-size: 15px;\">Yes, dedicated mentor support is available throughout the course. You can reach out for doubt-clearing sessions, project guidance, and career advice related to CRISPR &amp; Gene Editing. Our mentors are industry experts and experienced professionals.<\/div>\n<\/div>\n<\/div>\n<div style=\"margin-bottom: 30px; font-size: 15px; color: #2d3748;\">Enroll in A Hands-On Guide Using CRISPRdirect and CHOPCHOP today and take the next step in your professional journey. With expert-curated content, practical projects, and industry-recognized certification, this course is your gateway to mastering CRISPR &amp; Gene Editing skills that matter.<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>A Hands-On Guide Using CRISPRdirect and CHOPCHOP is a intermediate-level, 3 Days (1.5 hours per day) online course by NSTC. Master key concepts and practical skills in CRISPR &amp; Gene Editing through hands-on projects, real-world case studies, and expert mentorship. Earn your e-Certification + e-Marksheet upon successful completion.<\/p>\n","protected":false},"featured_media":109865,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"_members_access_role":[],"_members_access_error":""},"product_brand":[9487],"product_cat":[6253],"product_tag":[13138,14603,14607,14604,14602,6969,14606,14605,4757,14608],"class_list":["post-109866","product","type-product","status-publish","has-post-thumbnail","product_brand-nstc","product_cat-biotechnology","product_tag-biotechnology-course","product_tag-chopchop","product_tag-crispr-bioinformatics","product_tag-crispr-guide-rna","product_tag-crisprdirect","product_tag-gene-editing","product_tag-genome-editing-tools","product_tag-grna-design","product_tag-molecular-biology","product_tag-target-sequence-analysis","post-wrapper","thrv_wrapper","first","instock","sale","downloadable","virtual","taxable","purchasable","product-type-simple"],"acf":[],"_links":{"self":[{"href":"https:\/\/staging.nanoschool.in\/wp\/wp-json\/wp\/v2\/product\/109866","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/staging.nanoschool.in\/wp\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/staging.nanoschool.in\/wp\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/staging.nanoschool.in\/wp\/wp-json\/wp\/v2\/comments?post=109866"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/staging.nanoschool.in\/wp\/wp-json\/wp\/v2\/media\/109865"}],"wp:attachment":[{"href":"https:\/\/staging.nanoschool.in\/wp\/wp-json\/wp\/v2\/media?parent=109866"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/staging.nanoschool.in\/wp\/wp-json\/wp\/v2\/product_brand?post=109866"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/staging.nanoschool.in\/wp\/wp-json\/wp\/v2\/product_cat?post=109866"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/staging.nanoschool.in\/wp\/wp-json\/wp\/v2\/product_tag?post=109866"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}